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Information on phenotypes and genotypes of C. a/b/cans isolates obtained from Asian populations is sparse. Hence, the main aim was to determine the proportion of people colonized with C. a/b/cans in their mouths, among children, adult and elderly volunteers selected from the Colombo district and to find out if there are any phenotypic and genotypic variations in C. a/h/cans in three different age groups. We applied randomly amplified polymorphic DNA (RAPD) genetic typing technique, and a (commercially available) phenotyping technique for strain delineation of C. a/hi cans. A total of 120 healthy individuals in three different age groups (<6 years, 30-50 years and >70 years) were sampled by oral rinse method. Twenty-nine isolates of C. a/b/cans were identified by Gram staining, germ tube and API 20CAI]X test. Biotypes were determined using their ability to assimilate a variety of different sugars, and their resistance to boric acid For genotyping, DNA was extracted and fingerprinted with the RAPD technique using two random primers. This technique is versatile, simple and reproducible and has been previously used for typing candida. Forty one percent of the volunteers carried yeast in their mouths. The proportion of C. a/b/cans carriage was greater (23%) than that for other Candida species. There was a significant difference (p<O.O 1) between the recovery rate of yeast and C. albicans within the three age groups. On biotyping, 7 types were identified while RAPD analyses revealed 10 different genotypes. When the genotyping and the biotyping profiles were combined, a total of 18 composite types emerged. Both the RAPD and the phenotyping system we have established to type C. a/b/cans yielded reproducible results. Further investigation using the above methods and other epidemiologic typing techniques will be necessary to enhance the understanding of the epidemiology and pathogenesis of candidiasis.

The Phenotypic and Genotypic Diversity of Oral Candida albicans Isolates in Sri Lankan Cohorts of Varying Age Groups