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Objective: Mulberry leaf contains Vitamin C, Flavonoids, Oxyresveratrol and Saponin, which accelerated reepithelialization and increases wound contraction in burn healing. This study was designed to asses the effect of mulberry leaf extract ointment (Morus Alba L) on number of fibroblast and collagen fraction area in second degree burns.Materials and methods: This was an experimental design for 6 days with 24 male in Sprague dawley rat randomly selected and divided into 4 groups. The back of Sprague dawley male rats were shaved and given burns using 4 x 2cm iron plate which was put in 98o boiling water for 5 minutes and pasted for 10 seconds and smeared with distilled water, and ointment of mulberry leaf extract. Group I was a positive control (silver sulfadiazide ointment), Group II was a negative control (ointment base), Group III (mulberry leaf extract ointment 10%) and Group IV (mulberry leaf extract ointment 20%). The number of fibroblasts and collagen was evaluated with staining of hematoxylin eosin and staining mallory trichrome.Result and discussion: The mean of fibroblasts I, II, III,and IV was 21.33 ± 1.74, 13.28 ± 1.48, 17.56 ± 1.75, and 23.11 ± 2.38, respectively. The mean collagen fraction area I, II, III, and IV was 21, 11±7,42 , 16,18 ±7,72, 26,078 ± 8, 00 , and 25,79±8,33 respectively. One way Anova followed LSD test showed significant different in mean of between each treatment group obtained significant differences (p &lt;0.05). The mean number of fibroblast in the 10% and 20% ointment extract were significantly different compared with negative controls. Collagen fraction area in treatment group with 10 and 20% ointment were significantly different from that of negative control group, and was not significantly different from silver sulfadiazine groupConclusion: The mulberry leaf extract can be an alternative treatment for second degree burnsInternational Journal of Human and Health Sciences Vol. 05 No. 02 April’21 Page: 235-239

Potential Healing Effect Of Mulberry Leaf Extract Oinment on Burn Injury in rats