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The subfamily Diamesinae consists of the monogeneric tribes Boreoheptagini and Protanypini and the diverse tribe Diamesini with seven genera. These midges are prevalent in clean, cool arctic-alpine waters, but less abundant in the lowlands; therefore, the keys and descriptions to the known species of these four genera herein may prove valuable in the biomonitoring of these cool aquatic habitats. Larvae of this subfamily have squarish heads bordered by a wide, dark, postoccipital margin and dark mouth parts, short antennae, and have small to medium bodies, usually less than 10 millimeter long and with dark claws on the posterior parapod. The larvae of Protanypus saetheri Wiederholm is described for the first time. This identification is based upon the absence of other species in the collection area. Based on a detailed examination of a series of larval D. incallida Walker collected in Wyoming and a review of pertinent literature from Germany and Italy, it appears that there is considerable variation in the procerus and the labral armature. Because these characters are often used in keys, this variability can lead to misidentification of less recognizable species of Diamesa.

A guide to the larvae of the Nearctic Diamesinae (Diptera; Chironomidae), the genera Boreoheptagyia, Protanypus, Diamesa, and Pseudokiefferiella