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1 Efficiency of the technique to extract DIC thermodynamic Accuracy and precision of 613C determination of dissolved Accuracy and precision of 613C determination of DIC Effect of sulfate concentration on accuracy and precision of 613C determination of DIC extracted by the different Reliability of sampling devices stored under normal Effect of storing sampling devices under laboratory Effect of storing sampling devices in CO2 atmosphere under Effect of storing sampling devices under vacuum in Introduction 2 Theoretical Investigation 5 The precipitation technique 5 Efficiency of the technique to extract DIC 5 Accuracy of the technique for 613C measurements of DIC 6 Sample collection and sampling devices sources of error 8 Laboratory preparation sources of error 8 The gas evolution technique 10 calculations 10 Accuracy of the technique for 613C measurements of DIC 10 Sample collection and sampling devices sources of error 11 Laboratory preparation sources of error 12 Vapor phase equilibration: A proposed technique 12 Experimental studies 14 inorganic carbon (DIC) 14 Precision and accuracy of the isotope ratio mass spectrometer 15 Precision of 613C analytical procedure 15 extracted by the different techniques 16 The precipitation technique 16 The gas evolution technique 17 techniques 17 The precipitation technique 18 The gas evolution technique 19 Reliability of sampling devices during shipping and storing 19 laboratory conditions for one to two days 20 The precipitation technique 20 The gas evolution technique 20 conditions for two weeks 21 The precipitation technique 21 The gas evolution technique 22 a bell jar for one week 22 a bell jar 22 Effect of storing sampling devices at 60°C 23 Page Evaluation of sample treatment and laboratory preparation on 0-3C values 23 Effect of addition of HgC12 to water samples 23 Effect of filtering and(or) drying SrCO3 precipitate in the air 24 Effect of phosphoric acid concentration 25 Effect of purification on Cu and Mn02 furnaces at 450°C 25 Comparison between carbon isotope analysis of various natural waters obtained by the precipitation and gas evolution techniques 25 Conclusions 27 References 29

Methodologies for extraction of dissolved inorganic carbon for stable carbon isotope studies: Evaluation and alternatives