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Growth of Spirulina platensis requiers nitrogen, phosphate, and carbon. Urea is used as source of nitrogen, TSP as source of phosphate, and sodium bicarbonate (NaHCO 3 ) as source of carbon.  The purpose of this research was to evaluate C:N ratio of medium using different concentration of molase and urea addition as replacement of commercial medium. Spirulina platensis was cultivated  as a control consist of 1500 ppm NaHCO 3 as source of carbon, 80 ppm urea as source oh nitrogen, 20 ppm TSP (Triple Super Phosphate) as source of phosphate, 1 ppm FeCl 3 and 50 µg/l B12 vitamin as source of micronutrients. Spirulina platensis was cultivated in 2-liter of erlenmeyer flask with variation composition of urea and molasses in growth media by adding 20% (w/w) control medium. Urea was varied between 0; 16; and 80 mg L -1 , molasses was varied at the level of 0,1-0,9 g L -1 . Cultivation was maintained in pH 9-10, light intensity 6000-7000 lux and 28 0 C. Mini aerator pump is used as a medium stirrer. The best result were obtained at the medium varied of 0,1 g L -1 and 16 ppm of urea with growth rate of  0.230 day -1 , C:N ratio 6.8, and dry biomass of 0.333 g L -1 .

Evaluation of C/N Ratio in Spirulina platensis Cultivation using Molasses Addition as Organic Carbon Source