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ELISA represents a useful rapid method to detect the presence of specific antibodies on serum, plasma or meat juice collected at slaughter, however, false- and positive-results may occur depending on the sensitivity and specificity of the test. In this study we compare two commercial ELISA kits for the detection of specific antibodies against Trichinella spp. with respect to the gold standard method (artificial digestion) in a pig slaughterhouse. A total of 709 Iberian pigs belonging to 79 free-range herds were randomly selected and sampled (five to ten animals/herd) (Win Episcope 2.0; 95% confidence level, 8% accepted error). Blood samples were collected at the slaughterhouse, and serum was harvested and frozen at -80 oC until testing. Sera samples were analyzed for the detection of specific anti-Trichinella spp. antibodies by means of two commercial ELISA kits, following manufacturer’s instructions (PrioCHECK ® Trichinella Ab, Prionics; ID Screen ® Trichinella Indirect, IDVet Innovative Diagnostics; cut-off > 15% and > 50%, respectively). Samples from the diaphragm pillar (1 gram/animal) were collected and subjected to artificial digestion method for pooled sample digestion (100 g/pool) following the regulation EC-2075/2005. Specific anti-Trichinella spp. antibodies with PrioCHECK ® ELISA were detected in 3 out of 709 animals, belonging to 3 out of 79 herds. Nonetheless, all the sampled animals displayed negative results for both IDScreen ® ELISA and artificial digestion. The positive results observed with the first ELISA may be related to a higher sensitivity, being able to detect contact but not infestation with the parasite. Although both ELISA kits are coated with Trichinella E/S antigen, differences in the preparation and purification of the antigen may be related to different sensitivity and specificity. For this reason, serological tests are only recommended for surveillance studies, whereas direct methods should be used for food safety purposes.

Comparison of two commercial ELISA kits and magnetic stirrer method for detection of Trichinella spp. in a pig slaughterhouse