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POSTER PRESENTATIONs Discussion: A host specific Salmonella Choleraesuis remains the prevalent serovar in Poland and some other EU Candidate Countries as well as in the US (Hoszowski A. and Wasyl D., 2002;Weide-Botjes, M. et al., 1996). S. Choleraesuis var. Kunzendorf is responsible for the majority of the clinical salmonellosis in swine including acute septicaemia and pneumonia in piglets and fattening pigs. It causes up to 80% of Salmonella infections and more than 90% cases of clinical disease in Poland. The aim of present study was to determine the genetic relatedness of recent S. Choleraesuis isolates. The tested strains belonged to two clonal lineages. One of them (II) was represented by single strain isolated in 2000 and it was not found in pigs any longer. The other line spread over three years within animal population. High similarity of macrorestriction profiles and ribotypes indicate the affinity of the strains (Weide-Botjes, M., Liebisch, B., Schwarz, S., and Watts, J. L., 1996) and suggest clonal spread of the pathogen among swine in Poland. Some shift of predominant macrorestriction profiles and ribotypes was noted. Sub-cluster (I’) gathering mostly strains of Ch/X01 and Ch/X02 profiles predominated during first part of the three-year period. The second sub-cluster (I’’) covered isolates showing mostly Ch/X03 profile was more often found in 2002. All isolates presenting R-2 and R-3ribotypes were obtained in 2000. Either the macrorestriction profiles and the ribotypes revealed limited number of band differences that suggest that only a few genetic events took place during the spread of the clone (Tenover F.C. et al., 1995). The findings emphasise conservative character of S. Choleraesuis. Further long-time studies are needed to verify that thesis as well as to point out the possible crucial role of symptomless carriers or subclinical infected adult pigs for the disease spread among herds.

Comparison of two commercial ELISA for the diagnosis of salmonellosis in swine