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ORAL PRESENTATIONS Discussion and Conclusions: Among genotyping methods, PFGE has been shown to have superior discriminatory power and reproducibility. Currently, it is the gold standard fingerprinting technique used for subtyping foodborne bacterial pathogens in humans under the pulsenet system (CDC). AFLP has also, in recent years, gained popularity due to its high discriminatory power and reproducibility. However, these methods are also known to have a high initial cost and moderate ease of use (Gebreyes, 2003). Based on phenotypic classification of isolates in the present study, the most common and widespread cluster type (cluster type 11) was composed predominantly of phage type DT104. The genetic diversity within DT104 in the present study was limited as compared to other phage types. This is consistent with previous reports. Using antibiotyping as an alternative approach, two predominant pentaresistance types among 12 antibiotypes have been detected: AmCmStSuTe and AmKmStSuTe patterns (Gebreyes and Altier, 2002). The use of phage typing in addition to genotypic approaches remains an important step in subtyping Salmonella, particularly in outbreaks involving serovars Typhimurium and Enteritidis as recommended previously (Ward et al., 2001).

Campylobacter Prevalence and Diversity in Antimicrobial Free and Conventionally Reared Market Swine