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The objective of this study was to investigate the association of single nucleotide polymorphisms (SNPs) in innate immune response genes with Salmonella shedding in nursery pigs. One hundred and sixty eight pigs on seven farrow-tofinish farms and one farrow-feeder operation were included in the study. On each farm, 21 pigs were selected from seven sows at weaning. Fecal samples were collected from selected pigs and cultured for Salmonella, and the isolates were serotyped. DNA was extracted from liver samples and used to genotype pigs for single nucleotide polymorphisms (SNPs) in 21 different innate immune response genes. In total, 15 (9.3%) pigs tested positive for Salmonella; the isolates from six pigs from four different litters on one farm were serotyped as Salmonella Infantis and from nine pigs from six different litters on another farm as Salmonella Worthington. SNP analysis showed an association of Salmonella shedding with a SNP in the genes encoding mannan-binding lectin (MBL)-associated serine protease-2 (MASP-2) and Toll-like receptor-1 (TLR-1) (P < 0.05). These findings suggest that Salmonella shedding in pigs is controlled by genetic elements and these genetic variants could possibly be used to breed pigs that are more resistant to Salmonella colonization and Salmonella shedding. Introduction Salmonella is estimated to cause more than 1.2 million illnesses each year in the United States, with more than 23,000 hospitalizations (the leading cause of hospitalization due to foodborne illnesses), and 450 deaths (Scallan et al., 2011). In Canada, it is estimated that there are 88,000 human cases of salmonellosis annually (Thomas et al., 2011). Painter et al. (2013) have recently estimated that 46 % and 22 % of annual gastroenteritis cases associated with foodborne outbreaks in the United States are attributed to produce (fruits, nuts, and vegetable) and meat (beef, pork, and poultry) commodities, respectively. Asymptomatic pigs play a significant role in the spread and transmission of Salmonella from farm to fork. In fact, a major food safety concern is that Salmonella can be transmitted from pig farms to pork products at slaughterhouses, as well as to ground water and produce if the pig manure is used as land fertilizer. Despite a variety of suggested control measures for Salmonella, asymptomatic carrier pigs are very difficult to eliminate. As such, genetic selection for resistance against a Salmonella carrier-state could be utilized as an effective method to control Salmonella in pigs. However, the genetic markers causing the variation in response to Salmonella colonization and shedding, as part of the innate, cell-mediated and/or antibody mediated responses among pigs are still unknown. The objective of this study is to identify if single nucleotide polymorphisms in genes involved in pattern recognition and innate immunity are associated with altered Salmonella colonization and carrierstate status. Material and Methods Eight swine farms (7 farrow-tofinish farms and one farrow-feeder farm) in southern Ontario, Canada were included in this study. On each farm, 21 piglets were selected from 6-7 sows at weaning; in total 168 piglets (21 piglets per farm) were selected from 55 sows. Fecal samples were collected for Salmonella culturing at weaning. The piglets were euthanized at 5 weeks post-weaning and tissue samples collected from

Single nucleotide polymorphisms (SNPs) in genes related to innate immune response against Salmonella in nursery pigs