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Th1s field study was designed to evaluate the use of a live-attenuated Salmonella Typh1murium vaccine in pigs 1n respect of efficacy agamst S. Typhimurium at time of slaughter and the effect on serological herd monitoring using a commercial m1xed LPS-ELISA. About 1289 slaughtered p1gs (805 of non vaccinated groups and 484 of vaccinated groups) were investigated by bacteriological and serological examination (1149 p1gs). The study showed the efficacy of an oral vaccination with a live-attenuated Salmonella Typhimunum vaccme m reducmg the number of Salmonella carrymg p1gs at slaughter without a detectable interference with the serological monitoring of Salmonella (using a cut off at 40% 0 0 level). Introduction The work carried out in this study was designed to evaluate efficacy of a live Salmonella Typh1murium vaccme 1n a p1g population w1th low prevalence of Salmonella Typhimunum and no clinical signs of salmonellosis. Furthermore the impact of the vaccination on the monitoring of the herd w1th a commercial ELISA k1t test was investigated. Material and Methods The study was conducted as sequential trial in a commercial pig herd with no signs of clin1cal salmonellosis but subclinical infect1on with Salmonella Typh1murium. The swine population was a fattening one with p1glet production and reanng under "all-in-all-out"management. Dunng the first period of this study 805 nonvaccinated p1gs were sampled at slaughter At the same time sows and their offspring were vaccinated. Thereafter 484 vaccinated pigs were examined m the same way. The vaccine used was a commercial available live Salmonella Typhimurium vaccine (SALMOPORC®) and was based on a double-attenuated stram of S. Typh1murium, phage type DT 9, containing the serotype-specific plasmid of 60 MDa. Th1s stra1n can be distinguished from field stra1ns of the same serotype on the bas1s of its auxotrophy (adeand h1s-), using a rapid test (Salmonella Diagnostic Kit from lOT GmbH) and molecular b1ology methods (Schwarz et al., 1995). As far as possible all sows were g1ven one dose of the vaccine(~ 5 x 108 cfu) at 6 and agam at 3 weeks ante partum by the subcutaneous route. All v1able p1glets of the vaccinated sows were given a single dose by the oral route on day 21 and 42 post partum. Culturing of ileocaecal lymph nodes was done to demonstrate the presence of Salmonella (qualitative) us1ng standard m1crob1olog1cal culturing. Bacteriological culture carried out by 18 hours pre-enrichment at 37o C Buffered Peptone Water (BPW· Merck), 48 hours selective enrichment at 37o C 1n RVS med1um (Merck), with subculturing on to Rambach and XL T4 agar (Merck) plates after 24 and 48 hours culture. The plates were mcubated for 24 hours at 37o C and suspect colon1es confirmed by standard biochemical and serological tests (SIFIN, Berlin) The Wild-type stra1n distinguished from the vacc1ne stra1n us1ng the lOT Salmonella D1agnost1c Kit Blood samples were exammed by a Mixed-LPS-ELISA (SALMOTYPE®, Labor Diagnost1k, Le1pz1g) us1ng a cut off at 40% 00 level. Results In the bacteriological examination 64 of 805 non-vaccinated p1gs showed Salmonella Typhimurium at slaughter whereas 10 of 484 of vaccmated p1gs d1d so (Table 1 ). All isolates of the vaccmated Sos 1on 4: Control stratcg1cs Safepork 2007Verona (Italy) 237 pigs have been wild strains. The Odd Ratio for being tested positive in bacteriological examination for non vaccinated pigs was 4.09 (ci95%: 2.059.03, p=3.38E-6). The risk factor was not being vaccinated. The result suggested that vaccination reduced the number of Salmonella carrying pigs at slaughter. The serological examination of blood samples taken at slaughter revealed 127 of 802 pigs tested positive among the non-vaccinated groups and 55 of 347 pigs tested positive of the vaccinated groups. The OR for being tested positive in ELISA at 40% 00 level was 1.0 (ci95%: 0. 70 1.44, p=1 .0). The results of the serological examination showed that the oral vaccination didn't increase the number of pigs tested positive by ELISA. Table 1: Bacteriolo9ical and Serolo9ical results amon9 vaccinated and not vaccinated ei9s No Lymph tested Rate (%) of No No positiv Rate (%)of Date Group nodes positiv pos. lymph Serum samples pos. serum tested node sam~les sam~les (40%00} sam~les 2407 non-vaccinated 165 10 6.06 160 45 28.13 2108 non-vaccmated 100 3 3.00 92 26 28.26 2011 non-vaccinated 69 3 4.35 100 7 7.00 0412 non-vaccinated 118 1 0.85 100 16 16.0

The use of a Salmonella Typhimurium live vaccine to control Salmonella Typhimurium in fattening pigs in field and effects on serological surveillance