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Six sheep were used in two replications of a 3x3 Latin square design to compare the effects of dietary sources of conjugated linoleic acid (CLA) on availability of CLA and other long-chain fatty acids for absorption from the intestine. The three diets chosen to test these effects were a basal (control) diet, which contain 45% alfalfa hay, 50% cracked com, and 5% soybean meal, the basal diet plus 1 % CLA as the free acid, and the basal diet plus 1 % CLA as the calcium salt. Each period of feeding lasted three weeks, during which feed, rumen, duodenal, and fecal samples were collected to determine apparent digestibility of diet components, biohydrogenation of CLA in the rumen, and intestinal absorption of CLA and other long-chain fatty acids. It was found that dietary supplementation of CLA had no significant effect on dry matter and crude protein digestibility but increased lipid digestibility. Dietary CLA had no significant effects on ruminal VF A distribution. The availability of CLA for intestinal absorption and the intestinal absorption efficiency of CLA were increased significantly by supplementing the diet with CLA. About 96.7% of CLA fed as the free acid was biohydrogenated in the rumen, whereas feeding CLA as the calcium salt decreased biohydrogenation to about 90.1 %. Thus, feeding the calcium salt of CLA is a better way to increase availability of CLA for intestinal absorption than feeding CLA as the free acid.

Feeding to increase availability of CLA for absorption from the intestine of sheep and to increase CLA content in milk