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Oily surface films are of common occurrence in intensive larviculture of many fish species; these films are regarded as the cause for noninflation of the gas bladder. A method for sampling and quantifying oil surface microlayers in larval fish culture tanks is described. A glass plate was used to sample oil from the surface of tanks and the solvent 1 , 1 , 1 trichloroethane (TC E) used to separate the oi I from water. A spectrophotometric procedure was used to quantify the concentrations of oil in TCE by comparison with standard curves prepared with a food-grade cod oil.

Origin, characterization and mitigation of oil films in tanks used for intensive culture of larval walleye