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vin I. LITERATURE REVIEW 1 A. Reported History' 1 B. Fungal Characteristics 2 C. Isolation 3 D. Purification 9 E. Chromatography 10 F. Structure 10 G. Synthesis 11 H. Salivation Delay 12 I. Activation of Slaframine 16 J. Similarities to Acet\icholine 17 K. Active Metabolite 20 L, Review of Animal Studies 21 M. This Contribution 34 II. MATERIALS AND METHODS 36 A. Growth of the Organism 36 B. Extraction, and Purification of Slaframine 36 , i^reparation or me fiuorcbc^iHuic lycrivaiivc lyji D. HPLC Specifications for Fluorescamine Derivative Analysis 39 E. UV Spectral Anaiysis 39 F. Preparation of the Dose 40 G. Insertion of Catheter 40 H. Dosing the Animals 41 I. Collection of Blood Samples 41 J. Collection of Milk Samples 42 c A IS.. ^wOllCULiUi! ui Odiiva oaiiipic:> L. Method for the Extraction of Plasma 43 M. Method for the Extraction of Milk 44 N. Method for the Extraction of Saliva 46 O. Statistics and Computer Analysis 47

Pharmacokinetic study of slaframine in lactating goats and transfer into milk