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Hybridomas secreting monoclonal antibodies against three isolates of barley yellow dwarf virus (BYDV) were established. Two monoclonal antibody preparations were generated against the MAV isolate, one against RPV and six against P-PAV. None of the monoclonal antibody preparations reacted with healthy host componentsReactions of monoclonal antibodies, or unlabelled polyclonal antisera, in an indirect enzyme-linked immunosorbent assay (ELISA) indicate the presence of a common epitope on all three virus isolates. BYDV dissociated when incubated in carbonate-bicarbonate coating buffer at pH 9.6; stabilization was achieved by predialysis with either 2% formaldehyde or 2% glutaraldehyde. In indirect ELISA, unlabelled polyclonal antisera bound to both stabilized and dissociated homologous and heterologous BYDV isolates. However, conjugated polyclonal antisera were incapable of binding to any dissociated isolates or to stabilized heterologous isolates. Experiments with monoclonal antibodies in competition ELISA indicated the presence of at least two epitopes on the coat protein of P-PAV.

Production and use of monoclonal antibodies for molecular characterization, affinity purification, and serological detection of economically significant plant viruses