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viii CHAPTER I. GENERAL INTRODUCTION I CHAPTER 2. LITERATURE REVIEW 4 CHAPTER 3. EVALUATION OF GENETIC VARIABILITY IN, AND EXPRESSION OF, spliA AND sphB IN Leptospira borgpetersenii SEROVAR HARD JO . 20 CHAPTER 4. EVALUATION OF RECOMBINANT PROTEIN VACCINES CONTAINING PORTIONS OF SPHA AND SPHB IN A HAMSTER MODEL OF L borgpetersenii SEROVAR HARDJO INFECTION 84 CHAPTER 5. GENERAL DISCUSSION 99 APPENDLX A. ADDITIONAL FIGURES AND TABLES 103 .APPENDLX B. PROTOCOLS 108 REFERENCES 122 ACKNOWXEDGMENTS 143

Genetic variability and expression of sphA and sphB genes and immunogenicity of SphA and SphB of Leptospira borgpetersenii serovar hardjo