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iv GENERAL INTRODUCTION 1 Dissertation Organization 3 LITERATURE REVIEW 5 History of PRRS 5 Etiologic agent of PRRS 5 Biochemical and physical properties of PRRSV 7 Genome organization and replication 7 Viral proteins 9 Genotypic and antigenic diversity among PRRSV isolates 12 Epidemiology 14 Pathogenesis 16 Virus-cell Interactions 20 Immune response 21 Diagnosis of PRRS 23 Control and prevention 27 Conclusion 30 CELL SURFACE EXPRESSION OF VIRAL PROTEINS OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN INSECT CELLS AND THEIR ROLE IN PROTECTION AGAINST RESPIRATORY DISEASE 32 Abstract 32 Introduction 32 Materials and Methods 34 Results 37 Discussion 40 Acknowledgments 42 References 42

Analysis of viral proteins of porcine reproductive and respiratory syndrome virus in host protection and early virus-cell interactions