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13 INTRODUCTION 13 MATERIALS AND METHODS 15 Materials 15 In Situ Hybridization 15 RNA Blot Analysis 16 RESULTS 17 DISCUSSION 18 REFERENCES 20 CHAPTER 2: STRUCTURE OF THE CACI GENE AND IN SITU CHARACTERIZATION OF ITS EXPRESSION: THE ARABIDOPSIS THALIANA GENE CODING FOR THE BIOTIN-CONTAINING SUBUNIT OF THE PLASTIDIC ACETYL-CoA CARBOXYLASE 33 ABSTRACT 33 INTRODUCTION 34 MATERIALS AND METHODS 35 Materials 35 Isolation and Characterization of Macromolecules 36

Expression of genes encoding acetyl-CoA carboxylase, biotin synthase, and acetyl-CoA generating enzymes in Arabidopsis thaliana