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Five experiments were conducted to evaluate the use of plasma urea N (PUN) concentrations as a rapid response criterion to determine amino acid requirements. A preliminary experiment (Exp. 1) indicated that a 3-d feeding time is required to reequilibrate PUN concentrations after a change in the dietary concentration of lysine. In Exp. 2, 3, and 4, PUN was used to estimate the lysine requirement of growing pigs at different specific BW. Thirty individually-penned crossbred pigs weighing 32 and 44 kg in Exp. 2 and 3, respectively, were assigned to five dietary treatments (.60, .70, .80, .90, and 1.00% lysine) for 5 d. The PUN decreased quadratically (P < .05) to increasing dietary lysine. A two-slope, broken-line regression model estimated the requirement at .85% in Exp. 2 and at .76% in Exp. 3. In Exp. 4, 60 crossbred pigs (30 barrows and 30 gilts) weighing 70 kg were assigned to five dietary lysine concentrations: .50, .60, .70, .80, and .90% for 4 d. ^Reprinted with permission from 3. Anim. Sci. 1995. 73:472-481. %umal paper no. J-15806 of the Iowa Agriculture and Home Economics Experiment Station, Ames; Project 2919. ^The authors wish to express appreciation to Biokyowa, Chesterfield, MO for providing some amino acids used in this project. ^Supported by a Fulbright/Ministry of Education and Science (Spain) grant. ®To whom correspondence should be addressed.

Use of plasma urea nitrogen as a rapid response criterion to estimate the lysine requirements of growing and lactating pigs