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117 INTRODUCTION 118 MATERIALS AND METHODS 119 Construction of synthetic primers 119 Conditions for PCR 119 Amplification of bovine cDNA 120 Sequencing 121 RESULTS AND DISCUSSION 122 ACKNOWLEDGEMENTS 133 REFERENCES 134 GENERAL SUMMARY REFERENCES ACKNOWLEDGEMENTS 136

Studies on the role of expression of phosphoenolpyruvate carboxykinase gene in the development of bovine lactation ketosis