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53 INTRODUCTION 54 MATERIALS AND METHODS 59 Maize Lines 59 DNA Isolation 59 DNA Probes 60 Southern Blot Hybridization 61 Fragment Size Determination 63 RESULTS AND DISCUSSION 64 LITERATURE CITED 75 SECTION II: MOLECULAR CHARACTERIZATION OF shrunken MUTATIONS INDUCED IN MUTATOR LINES OF Zea mays L. 78 ABSTRACT 79 INTRODUCTION 80 MATERIALS AND METHODS 84 Plant Material 84 DNA Isolation and Southern Blot Hybridization 84 DNA Probes 94 Quantification of Mu Element Copy Number 97 RESULTS 99 All Mutator-induced shrunken Mutant Lines Contain Multiple Mul-like Elements 99 Molecular Lesions Associated with Mutatorinduced sh Mutant Alleles 104

The molecular characterization of mutations induced in Mutator lines at the Bronzel and Shrunken loci in maize