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17 INTRODUCTION 18 MATERIALS AND METHODS 20 Drosophila Strains 20 Basic Drosophila Techniques and Microinjections 20 Plasmid Constructioi\s 21 D-raf and Raf-1 Constructs 21 Site-directed Mutagenesis 21 Deletion Mutants 22 Expression of D-raf Proteins in Sf9 Cells 22 Phosphopeptide Mapping 22 Western Blot Analysis of Microinjected Embryos 23

The role of D-raf in the terminal class signal transduction pathway in Drosophila melanogaster