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8 INTRODUCTION 10 MATERIALS AND METHODS 11 RESULTS 14 DISCUSSION 18 REFERENCES 21 CHAPTER 11. REPLACEMENT OF Asp^^^ WITH Asn BY SITEDIRECTED MUTAGENESIS CHANGES THE SUBSTRATE SPECIFICITY OF ESCHERICHIA COLI ADENYLOSUCCINATE SYNTHETASE FROM GUANOSINE 5'-TRIPHOSPHATE TO XANTHOSINE 5'-TRIPHOSPHATE ABSTRACT 28 INTRODUCTION 29 EXPERIMENTAL PROCEDURES 31 RESULTS 32 DISCUSSION 3628 INTRODUCTION 29 EXPERIMENTAL PROCEDURES 31 RESULTS 32 DISCUSSION 36

Investigation of the GTP-binding consensus sequences in Escherichia coli adenylosuccinate synthetase and the enzyme's reaction mechanism