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and Implications Bovine mammary epithelial cells that were treated with bacterial lipopolysaccharide and 1,25-dihydroxyvitamin D3 showed an increase in the expression of the genes for inducible nitric oxide synthase (iNOS) and S100 calcium binding protein A12 (S100 A12). iNOS and S100 A12 are part of the innate immune response and expressed in the mammary gland during mastitis. Production of 1,25dihydroxyvitamin D3 in the mammary gland during mastitis, then, may be an important component of the innate immune response.

1,25-Dihydroxyvitamin D3 Enhances Bovine Mammary Epithelial Innate Immune Responses