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The chemical composition of three essential oils of Hyptis suaveolens was performed by gas chromatography coupled with mass spectrometry and their bioactivity was deduced from a multi-dose immersion test in increasing progression. The results show that the essential oils of H. suaveolens analysed are of two types: a sesquiterpenedominant type (51.86%) very rich in β-caryophyllene (20.69%) and a monoterpene-dominant type (38.08%) which is distinguished from the first by a relatively high eucalyptol (1.8-Cineole) content (12.11%). Laboratory tests indicate a high toxicity of the β-caryophyllene chemotype on females of R. (B.) microplus. Only gorged females exposed to the two concentrations (1% and 2%) of this oil laid eggs with a laying delay of 168 hours. The egg laying inhibition rate recorded for these two concentrations is 65 and 98% respectively. The essential oil of H. suaveolens therefore represents a very interesting alternative to the use of synthetic antiparasitic.

Bioactivity of essentials oils of Hyptis suaveolens (L.) on the reproductive parameters of the Rhipicephalus (Boophilus) microplus