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Haritakyadi eye drops are prepared by hydro-distillation (Arka kalpana) of ingredients like Terminalia chebula Retz. (Haritaki), Emblica officinalis Linn. (Amalaki), Berberis aristata Dc. (Daruharidra) and Glycyrrhiza glabra Linn. (Yastimadhu). The research was designed to explore the volatile constituents by Gas Chromatography-Mass Spectrometry (GC-MS). Secondary metabolite screening using GC-MS revealed the presence of fifteen constituents. Out of fifteen constituents 8 could not be identified and remaining 7 compound identified as 1,3,5,7-Cyclooctatetraene; Benzene, 1ethyl-4-methyl; Benzene, 1,2,3-trymethyl; 1,3-Xylyl-15-crown-4, 2,3-pinanedioxyboryl; Silane,[[9-[[(dimethyl-2propenylsily)oxy]methyl]6a,7,10,10a-tetrahydro-6,6-dimethyl-3-pentyl-6H-dibenzo[b,d]; Silane, dimethyl (3-phenylprop2-enyloxy)hexyloxy; Eicosanoic acid and tetradecyl ester at different retention time and peak area percentage. The compounds were identified using NIST library, two of them were silane were artifacts. This article reveals that Haritakyadi eye drops can be used for pharmacological activities.

Gas chromatography – Mass spectrometric analysis of Haritakyadi eye drops: A poly herbal compound for ophthalmia neonatorum