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The urinary excretion of 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been used as a biomarker of oxidative DNA damage in both the clinical and occupational setting. The urinary 8-OHdG in traffic policemen posted at busy traffic junctions were estimated along with the control population away from the busy traffic junctions those doing administrative job. A total of 105 urinary samples (60 samples of traffic policemen and 45 samples of control population) were collected for estimation of 8-OHdG and analyzed using enzyme linked immunosorbent assay (ELISA). The mean 8-OHdG was significantly higher (13.42±1.61) μg/g creatinine) than those of control group (9.34±1.36 μg/g creatinine) (p<0.05). The study showed that urinary 8-OHdG is associated with occupational exposure and other lifestyle factors.

Biomonitoring of Oxidative DNA Damage in Traffic Policemen Exposed to Urban Air Pollution