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For the corticotropin-releasing-hormone stimulation test the number of samplings and measurements are reduced to two--and the ratio between concentrations at 60 min and 0 min is calculated. The difference between the information given by absolute concentrations and ratio is negligible, but by using ratio, the influence of bias is eliminated. The test becomes simpler and the costs are reduced. The use of only two measurements facilitates the evaluation of quality specifications.

upsala journal of medical sciences

6.1.1.5 Biological Description of the Cortisol Responses to Corticotropin-Releasing-Hormone (CRH) Stimulation. An Optimization and Simplification of the Test