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A study was conducted to determine the effects of feeding Dried Broccoli Florets (DBF) to layers on egg yolk fatty acid deposition. Seventy-two layers were randomly allotted to four dietary treatments (six cage replicates with three hens each) and fed diets containing 0, 4, 8, and 12% DBF for 56 days. Results showed that inclusion of DBF decreased (linear effect, P< 0.001) concentrations of Saturated Fatty Acids (SFA) and increased (linear effect, P< 0.001) concentrations of poly-unsaturated fatty acids (PUFA). Egg yolk concentration of palmitic acid decreased (linear effect, P< 0.001) while linoleic (quadratic effect, P = 0.003) and linolenic (linear effect, P < 0.001) acid concentrations increased as the level of DBF in the diet increased. It was concluded that feeding DBF at 12% of the diet increased PUFA concentrations and decreased those of SFA. Introduction Broccoli (Brassica oleracea L. var. italica) is an important vegetable crop in Canada with an annual production of 32,000 tons. As with other vegetables, large amounts of broccoli wastes are generated during harvest, packaging and marketing. It has been estimated that about 40 to 50% of total broccoli produced is discarded during processing as a result of the high standards imposed by consumers and retailers, and due to consumer refusal at the retail level. Additional losses occur in the field, generating large quantities of florets, stems and leaves as crop residues. As broccoli production increases, there is a concomitant increase in the quantity of residues produced. These residues are often discarded into the environment where they pose major environmental concerns. There is growing interest in developing new feeds from waste vegetable such as broccoli by-products to replace conventional feeds. Recent research showed that broccoli residues such as broccoli leaves and stems and broccoli florets can be incorporated in layer diets to substitute conventional feeds such as soybean meal. Incorporation of dried broccoli leaves and stems up to 9% of the diets had no effect on egg production, but significantly improved egg quality with higher yolk xanthophyll and lower yolk cholesterol concentrations [1], In more recent study, [2] reported that feeding Dried Broccoli Florets (DBF) up to 12% of the diet had no negative effects on feed intake, egg production and feed efficiency and improve egg yolk color and α-tocopherol. Chemical analysis of different broccoli parts showed that florets contained higher CP (22.4%) but lower crude fiber (11.7%) concentrations than broccoli leaves and stems [3]. Broccoli florets are also a rich source of poly-unsaturated fatty acids which constitute 62% of the total fatty acids [3]. Inclusion of feeds rich in PUFA has been shown to increase n-3 PUFA deposition in egg yolks without compromising egg production [4–6]. To the best of our knowledge, no studies have investigated the effects of dried broccoli floret (DBF) residues on egg yolk fatty acid profile. Materials and Methods Birds and housing All animal procedures were approved by the Animal Care Committee of the Faculty of Agricultural and Environmental Sciences of McGill University. This study was part of larger study in evaluating the effects of feeding DBF on layer performance and total tract nutrient retention [2]. Preparation, processing and chemical composition were reported [2]. A Total of 72 (64-week-old) White Leghorn laying hens were weighed and placed in 24 cages (3 birds/cage) with six cage replicates. Each cage representing one replicate, was assigned to one of four experimental diets containing 0, 4, 8 and 12% DBF for 56 days. Dried broccoli florets partially replaced corn and soy bean meal (Table 2). All diets were formulated to be iso-caloric and iso-nitrogenous according to [7] and were offered in a mash form. Feed and water were provided ad libitum. Birds. Birds received equal daily lighting time (16L: 8D) at constant room temperature. Sample collection Feed intake was measured by-weekly. Two eggs from each cage replicates were collected at random, cracked, and yolks were separated from the whites. Samples of pooled egg yolks (2 eggs/cage replicates/ treatment) for each treatment were collected at week 2, 4, 6, and 8 of the experiment (n = 48). The yolk samples were frozen at −20 °C, freeze dried, and finely ground before fatty acid analysis. Methyl esters of fatty acids were prepared from yolk, feed, and DBF samples according to [8]. Acid composition of methyl esters was determined by gas chromatography as described by [6] Statistical Analysis Data were analyzed using the PROC MIXED procedure [9] with the following model: A.F. Mustafa (2019) Feeding Broccoli Floret Residues on Layers: II. Effects on Fatty Acid Deposition Integr J Vet Biosci, Volume 3(1): 2–4, 2019 Yijk = μ + Ti + Cij + eijk Where: Yijk= observation, μ = overall mean, Ti= fixed effect of i th treatment (i= 1, 2, 3 or 4), Cij= random effect of j th cage within ith treatment (j = 1, 2, 3, 4, 5 or 6), eijk= residual error (k = 1 or 2), eijk ∼ N (0, σ 2e). The least significant difference method was used to identify statistically different means (P <0.05). Orthogonal contrasts were used to test for linear and quadratic effects of adding DBF to the diet. The least square mean method was used to identify differences among treatment means and statistical differences were declared at p < 0.05. Data were analyzed by one-way ANOVA using the GLM procedure [9] with cages as experimental units. Least significant difference method was used to identify statistically different means (P < 0.05). Orthogonal contrasts were used to test for linear and quadratic effects of adding DBF to the diet. Results Linolenic acid (C18: 3n3) was the most abundant fatty acid followed by palmitic and oleic acid, respectively (Table 2). Dietary C18: 3n3 increased by 8.2, 24.5, and 61.2% as BDF increased by 4, 8, and 12%, respectively (Table 1). Saturated (SFA) and mono-unsaturated (MUFA) fatty acids of egg yolk decreased (linear effect, P < 0.0001) with increasing dietary DBF (Table 5). The reductions in SFA and MUFA were mainly due to the declines in palmitic (quadratic effect, P = 0.0838) and oleic (linear effect, P < 0.0001) acids, respectively. In contrast, poly-unsaturated fatty acid (PUFA) concentrations in egg yolk increased (linear effect, P< 0.0001) as the level of dietary DBF increased. Consequently, SFA: PUFA ratio decreased (linear effect, P< 0.0001) in yolks produced by layers fed DBF. As the level of dietary DBF increased, yolk linolenic acid content (quadratic effect, P = 0.011) with highest concentrations being achieved for layers fed 8 and 12% DBF diets. A similar increase in yolk linoleic (linear effect, P< 0.0001) content was also observed as the level of dietary DBF increased. Discussion Linolenic acid concentration of DBF constitutes 35.4% of the total fatty acids, which is consistent with the values reported for broccoli florets [9,10], The Fatty acid profile of egg yolk reflected the dietary fatty acid composition. The increase in PUFA and linolenic acid concentrations is likely due to the high levels of linolenic acid concentration in DBF-based diets (Table 1) and DBF (Table 2). Inclusion of DBF at 4, 8, and 12% of the diet were accompanied with significant increases (i.e. 23.3, 66.3, and 68.6%, respectively) in egg yolk deposition of linolenic acid when compared with the control diet. Feeding layers diets rich in linolenic acid such as cabbage residues [11], pasture [12], and flaxseed [4, 6] has been successfully used to increase the concentration of linolenic acid as well as other health promoting fatty acids in egg yolk. The lower egg yolk SFA and MUFA concentrations produced by layers fed DBF diets can be attributed to their lower concentrations in DBF diets and \ or the inhibitory effects of PUFA. It is well documented that PUFA inhibit the activity of 9∆ desaturase which is involved in MUFA synthesis [13, 14]. Conclusions It was concluded that incorporation of DBF in layer diets reduced egg yolk concentrations of SFA and increased those of PUFA. Greater deposition of omega-3 fatty acids (e.g. C18: 3n3) can be achieved by 6 or 9% DBF. Table 1. Ingredients and chemical composition of dietary treatments Broccoli floret residue inclusion (%)