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The objective of this research was to study the genotype and biotype of BVDV variability among PI dairy cattle in Java, Indonesia between 2016 and 2017. Two hundred isolated buffy coat from dairy cattles that had low reproductive performance and never been vaccinated in Java were used in this studies. Using antigen capture Elisa, 12 out of 200 dairy cattles were positive for the presence of protein Erns BVDV (6.0%). The PI status was confirmed by multiple sequential viral molecular detection. Through phylogenetic and nucleotide sequence analysis of the 5’-Untranslated Region (5’UTR) of the samples investigated, it was determined that all the 12 field positive samples had the BVDV-1 genotype. Three IP-BVDV positive samples (2282-15, 061014 and 0813-2) sharing highest similarity (99% homology) with subgenotype BVDV-1c AY763030-1 and KF896608 isolates from Australia. Using Immuno Peroxidase Monolayer Assay (IPMA) the biotype of all the samples were identified as noncytopathic-BVDV. All of the informations would be necessary for designing the diagnostic tool and/or vaccine that match the circulating BVDV subgenotype.

Genotypes and Biotypes Variation of Bovine Viral Diarrhea Virus from Persistently Infected Dairy Cattle in Java, Indonesia