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Growing environmental concerns and increasing demands from end-use sectors have increased the glob-al market for microbial products. Optimizations of production parameters hold great importance for the industry. The present study was aimed at optimization of submerged state fermentation conditions for production of lignocelluloly-tic enzymes from digested biogas slurry by Phanaerochaete chrysosporium MTCC 787. Enzyme activities for differ-ent enzymes i.e. endoglucanase, exoglucanase, I²-glucosidase; xylanase and mannanase; laccase, lignin peroxidase and manganese peroxidise, using P. chrysosporium MTCC 787 were maximum at 50% concentration of digested slur-ry and showed maximum value of xylanase i.e. 187.41U/ml. Effect of temperature (25Â°C, 30Â°C and 35Â°C) on lignocellu-losic bioconversion showed that at 30Â°C, maximum value of manganese peroxidise (167.5 U/ml) was obtained. High-est enzyme activites were obtained at selected inoculum size i.e. 10â·spores/ml, e.g. 85.29 U/ml xylanase was ob-tained. Incubation period of 8 days and pH of 7.0 came out to be best conditions for P. chrysosporium MTCC787 to produce maximum enzyme activity e.g. xylanase 95.47U/ml at pH 7.0 and xylanase 144.96U/ml at 8 day incu-bation.This work presents a novel concept in optimization of fermentation process to produce lignocellulolytic en-zymes as this work is focussed on utilization of digested biogas slurry as a substrate for enzyme production and enhancement of the production with microbial source, which is environment friendly.

journal of applied and natural science

Optimization of cultural conditions for submerged state fermentation of di-gested biogas slurry for production of lignocellulolytic enzymes using Phanaerochaete chrysosporium MTCC 787