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Dolichos biflorus, a commonly used legume in Uttarakhand, produces alpha amylase enzyme for conversion of starch present in its cotyledons to glucose, so that this glucose can be further utilized for the life controlling processes, glycolysis and Krebâ€™s cycle. Yield of this Ai - amylase isolated from the germinating legume comes out to be 27.7 IUml-1. Maximal amylase production occurs at pH 6.1 at 45O C. The enzyme was purified two fold, first with ultra-filtration and then with Ion-exchange chromatography. Ultra-filtration revealed size of amylase to be between 10 kDa and 30 kDa, against larger sizes of other bacterial amylases. The pH and temperature optima for purified enzyme were 6.1 and 45OC respectively. The Km for starch came out to be 1.95 mgml-1. This finding of generating one more new and low-price source of Ai - amylase is a great advancement in biotechnology.

Partial purification and characterisation of some low molecular weight á - amylases from Dolichos biflorus