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This study was aimed to investigate the use of Mustard stalk as a cheap, eco-friendly adsorbent with support matrix for the immobilization of microbial cell and for subsequent removal of 2,4-dichlrophenol(2,4-DCP) from waste water. A comparative batch study between adsorption as well as simultaneous adsorption and biodegradation (SAB) of 2,4-DCP by mustard stalk immobilized Pseudomonas putida MTCC1194 have been studied in conical flask having concentration ranges of 2,4-DCP from 100 to 1000 mg l -1 with adsorbent dose range 1 to 12 g l -1 at pH range 2 to 9 and temperature range 28 ° C to 35 ° C, placed in an orbital shaker. The results of the batch studies showed that simultaneous adsorption and biodegradation (SAB) shows the maximum percent (91%) removal of 2,4DCP as compared to simple adsorption (86 %) at optimum temperature 32 °C of adsorbent dose 10 g l -1 , and pH 6 with MSAC having particle size 0.24 mm. The equilibrium data for 2,4-DCP degradation sorbent systems were well fitted with Langmuir isotherm.

Removal of 2,4-dichlorophenol by simultaneous adsorption and biodegradation (SAB) using low cost adsorbents