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The present study focuses on the exploitation of sweet sorghum biomass as a source for hydrogen in continuous and batch systems. Sweet sorghum is an annual C4 plant of tropical origin, well-adapted to sub-tropical and temperate regions and highly productive in biomass. Sweet sorghum biomass is rich in readily fermentable sugars and thus it can be considered as an excellent raw material for fermentative hydrogen production. Extraction of free sugars from the sorghum stalks was achieved using water at 30°C. After the extraction process, a liquid fraction (sorghum extract), rich in sucrose, and a solid fraction (sorghum cellulosichemicellulosic residues), containing the cellulose and hemicelluloses, were obtained. Hydrogen production from sorghum extract was investigated using mixed acidogenic microbial cultures, coming from the indigenous sorghum microflora and Ruminococcus albus, an important, fibrolytic bacterium of the rumen. Hydrogen productivity of sorghum residues was assessed as well, using R. albus.

Biohydrogen production from sweet sorghum biomass using mixed acidogenic cultures and pure cultures of Ruminococcus Albus