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Pseudomonas syringae is a complex group of bacteria, which comprises nine different genomospecies and over 50 pathovars. Strains of P. syringae have been isolated from some rivers and lakes in New Zealand. To determine whether these waterways act as reservoirs of plant pathogenic bacteria, 15 strains of P. syringae isolated from the Waikato River and Whakapapanui stream have been further characterised using several polymerase chain reaction (PCR) protocols. Five of those 15 strains belong to genomospecies 1, which comprises P. syringae pv. syringae, but none belongs to genomospecies 2. The protocol for detection of P. syringae pv. papulans was modified and is now specific for this pathovar. The identity of a strain isolated from the Waikato River as being P. syringae pv. atrofaciens has yet to be confirmed. None of the 15 strains studied belongs to the pathovars papulans, actinidiae, tagetis, helianthii or theae.

proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference

The application of polymerase chain reaction for characterising strains of Pseudomonas syringae isolated from New Zealand rivers