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This paper presents the first report of cultivation of Scutellospora calospora with Ri-TDNA transformed carrot (Daucus carota. L) roots. Carrot root transformation was done using Agrobacterium rhizogenes. Spore germination tests were conducted on 6% water agar and minimal media to assess the time taken for germination. Morphological characteristics of extraradical mycelium, intraradical mycelium, branched absorbing structures and auxiliary cell formation were recorded. Auxiliary cell formation started within 3-5 days of root contact and continued until 2 months. There was an extensive, thick, brown extraradical mycelial development following root infection, but spore formation was low (four spores) and was observed only after 8 months. Spores were full of oil globules and were produced on MSR medium but not M medium.

proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference

Monoxenic culture of the arbuscular mycorrhizal fungus Scutellospora calospora and RiTDNA transformed carrot roots