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Sclerotium cepivorum sclerotia require incubation in soil to overcome constitutive dormancy, a condition where the sclerotia will not germinate even when stimulated. In Trial 1, artifi cial onion extract, diallyl disulphide (DADS) was used to stimulate sclerotial germination of laboratory produced sclerotia after 1, 2 and 3 month conditioning periods when incubated in two different soil types. The results showed that soil type and fungal isolate did not affect dormancy and that approximately 16, 33 and 21% of the sclerotia germinated after 1, 2 and 3 month conditioning periods, respectively. In Trial 2, DADS signifi cantly increased sclerotial germination compared with the control after 2, 3, 4, 5 and 6 month conditioning periods. Sclerotia required 6 months in soil before high rates of germination occurred (>89%) when stimulated. When a natural population of sclerotia (i 8 weeks old) (Trial 3) was exposed to DADS, 51% of the population germinated compared with 21% in the control (P<0.001).

proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference

Dormancy of Sclerotium cepivorum sclerotia in New Zealand soils