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Carrot seedlings are susceptible to damage from grass grub larvae. The biological control bacterium, Serratia entomophila, was applied to the surface of carrot seeds via pelleting or as a biopolymer seed coating and the activity against grass grubs determined in pot trials. Seedling mortality caused by grass grub larvae was signifi cantly reduced (P<0.05) in two trials from 88 and 64% in untreated pots to 26 and 13% in pots containing pelleted seed and 7 and 16% in pots containing biopolymer-coated seed. Shelf life studies showed formulations were stable at 4°C for at least eight weeks and for two weeks at 20°C, after which cell viability decreased over time. Bioassay results showed little difference between the two treatments, despite a higher concentration of bacteria on the biopolymer-coated than the pelleted seeds. The potential of seed coating as a delivery mechanism for biocontrol agents has been demonstrated and future possibilities are discussed.

proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference

Carrot seed coating with bacteria for seedling protection from grass grub damage