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Callas (Zantedeschia spp.), a plant native to south-central Africa, are an economically important export cut flower and tuber crop in New Zealand. Bacterial soft rot, caused by Erwinia carotovora (Ec), is the main disease of callas grown in New Zealand. Each year the disease causes substantial losses to plants in the field and to tubers in storage. Biochemical tests are currently the accepted standard for identification and taxonomy of soft rot erwinias, but on a routine level they are very time consuming and do not always provide a tentative identification. Pel genes encoding pectolytic enzymes are considered the main pathogenicity factors of soft rot erwinias. This study describes a rapid and sensitive PCR assay that is capable of specifically detecting pel genes present in Ec from healthy and diseased calla tubers.

proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference

Effects of bruising on development of bacterial soft rot in tubers of 22 potato cultivars