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A number of formulations of Btk (Bacillus thuringiensis kurstaki) are available in New Zealand for control of lepidopterous species. The efficacy of these formulations is often tested subsequent to spraying, using exposed host foliage in larval bioassays. To provide additional quality assurance, the standard American potency bioassay has been adapted by using third instar Teia anartoides larvae, a relevant pest species, and Dipel DF (a dry Btk formulation with a long and stable shelf life) as reference potency for comparison. A series of different batches of a Btk formulation were incorporated into artificial diet at rates known to be sublethal and in the region of LC50. Larvae were placed on to the diet and mortality assessed at 4 and 7 days. Control larvae were reared on diet without Btk. All formulation batches produced similar results to the reference (Dipel DF) with the exception of the oldest batch that had slightly lower potency. Potency testing using artificial diet can provide quality assurance for different batches and, combined with efficacy testing on host foliage, improve the auditing of spraying operations.

proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference

Measuring potency of Btk formulations against painted apple moth (<i>Teia anartoides</i>) larvae using an artificial diet bioassay