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Analysis of microbial communities associated with plants, insects and soil has been a significant challenge for plant protection researchers, because of the lack of techniques with which to access these populations. A new molecular community profiling technique, 16S rRNA gene-based PCR followed by denaturing gradient gel electrophoresis (DGGE), has been used to analyse microbial communities in a number of environments and has many potential uses in plant protection research. The technique is currently being used for the analysis of insect gut microflora, characterisation of phylloplane and rhizosphere microbial populations, and in environmental assessment of the effects of biopesticides and new technologies on indigenous soil microbes.

proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference

Denaturing gradient gel electrophoresis a tool for plant protection research