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Background: Ganoderma lucidum is a traditional medicinal mushroom that has many therapeutic applications. However, the application of this beneficial fungus has been limited due to its secondary metabolite’s low production. Many investigations have been carried out to improve the production of G. lucidum as well as Ganoderic Acid (GA); however, prior studies suffer from the lack of sufficient monitoring platform. A real-time monitoring study could be useful to find more information through cultivation and evaluating secondary metabolite production. Materials and Methods: In the current study, aerated shaken flasks (AF) with different culture mediums were used for online measurement of the respiration activity of G. lucidum in small scale bioreactors. Then, to find more information through G. lucidum growth, four factors, including biomass formation, GA, residual sugar, and gene expression were evaluated on different days. Results: Online monitoring of cell proliferation demonstrated that GA starts to synthesize in the second growth phase as a partially growth-associated metabolite. High maximum biomass and GA production were obtained at an initial glucose concentration of 35 g/L with vitamin and KH2PO4. During fermentation, the YO2/GA and Y O2/X the rate of oxygen consumption rate per GA production and biomass formation, respectively, were introduced as beneficial parameters to scale-up the process. Conclusions: A novel monitoring strategy was suggested which can be beneficial for future investigations.

Growth Kinetics and Ganoderic Acid Production from Ganoderma lucidum GIRAN17: A Real-Time Monitoring Platform