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Five-day seeded Biochemical oxygen demand (BOD) tests using Glucose-­Glutamic acid (GGA) substrate were carried out. The seeds used are a regular seed an NX seed and an over-the-counter food supplement commonly referred to as a probiotic. The purpose was to determine if seed type influenced 5-day BOD test results. The NX seed contained a chemical formulation designed to inhibit nitrification. Test results indicate that 5-day BOD varied significantly with each seed. Micrographs of stained samples prepared from cultures of the three seeds identified Gram-stain negative rods present in all the three inoculates.

Evaluation of two commercial inoculates and a probiotic food additive as seeds in BOD testing