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The Balkan Botanic Garden of Kroussia has formulated a conservation strategy for the collection and documentation of wild plant material for sustainable utilization, prioritizing the Greek rare threatened endemics. In this framework, the species studied were the endangered Erysimum krendlii Polatschek, the vulnerable Centaurea paxorum Phitos & Georgiadis and the rare Erysimum naxense Snogerup: all are range-restricted, local Greek endemics with small populations in the wild, therefore with conservation priority, showing also potential commercial interest (aromaticmedicinal properties, edible parts or as pot plants for gardening). The effect of the immersion of the base of the cuttings for 1 min in indole-3-butyric acid (IBA) solutions at different concentrations (0, 1000, 2000, 4000 ppm) on root formation was tested for all species. A peat moss: perlite substrate (1:3 ratio) was used for the experiment. E. krendlii had better performance with 4000 ppm IBA showing 100% rooting, 30.86 roots 3.71 long after 5 weeks in the mist. Rooting of C. paxorum cuttings was optimum after 3 weeks exhibiting 100% rooting, 47.86 roots 2.61 cm long with 1000 ppm IBA. E. naxense gave 85.71% rooting, 19 roots 3.06 cm long with 4000 ppm IBA after 8 weeks. All the young individuals produced were transplanted in bigger pots (0.33, 1 Lt) until their final transplanting pot size (2.5 Lt), each time in a peat moss: perlite: soil substrate (2:1⁄2:1⁄2 v/v), for their subsequent growth. The successful asexual propagation of the studied wild species allowed the creation of mother plants, facilitated their ex situ conservation and domestication.

gsc biological and pharmaceutical sciences

Vegetative propagation of three prioritized Greek endemics with potential commercial interest: Erysimum naxense Snogerup, Erysimum krendlii Polatschek and Centaurea paxorum Phitos &amp; Georgiadis