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This study is conducted to determine a steroid compound from the dichloromethane extract of matoa’s stem bark (Pometia pinnata) and toxicity assay against Artemia salina Leach. In this reseacrh, extraction was carried out by maceration, separation by chromatography, purification by recrystallization, and determination of molecular structure by spectroscopic method (UV-Vis, IR, and MS). Toxicity test against shrimp larvae Artemia salina was conducted by BSLT method. The steroid isolate was obtained as colorless powder with melting point of 147-148oC. Based on the spectroscopic data, it was identified as stigmasterol. Isolate showed toxicity against Artemia salina with LC50 of 41.334 μg / mL. Keywords— Stem bark of matoa, dichloromethane extract, BSLT.

Steroid Isolated from the Dichlorometane Extract of Matoars Stem Bark (Pometia pinnata) and Toxicity Tests Against Artemia salina Leach