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Emblic fruit wine rich in alcohol, phenolics. This experiment adopts the reverse high performance liquid chromatography (HPLC) method for emblic leafflower fruit wine representative of phenolics gallic acid, quercetin content determination of study; And the emblic leafflower fruit forint phenol method study on determination of total phenol content in fruit wine. Experimental results show that the forint phenol method is used to measure total phenolic content (with gallic acid) in the range of 1-6 ug/ml has good linear relation, measured over the basic total phenol content in fruit wine was 4.6 mg/ml; Reverse high performance liquid chromatography (HPLC) method to measure content of gallic acid, quercetin in 8-400 ug/ml has good linear relation, measured over the basic content of gallic acid in fruit wine was 0.82 mg/ml, quercetin is 0.94 ug/ml. KEYWORD: RP-HPLC, Phyllanthus emblica, wine, gallic acid, quercetin 4th International Conference on Mechanical Materials and Manufacturing Engineering (MMME 2016) © 2016. The authors Published by Atlantis Press 479 capacity. Water bath reaction under 66 degrees Celsius for 10 minutes, 760 nm wavelength determination of absorbance, establish standard curve. Sample measurement: precision measuring preparation good emblic leafflower fruit wine sample solution 0.5 ml in 50 ml volumetric flask and add 30 ml deionized water, shake well, add 2.5 ml forint phenol reagent, 8.0 ml9 % sodium carbonate solution, constant volume, deionized water and mixed water bath reaction under 66 degrees Celsius, 10 minutes after cooling determine its absorbance at 760 nm. Stability testing: accurate said emblic leafflower fruit wine sample solution 0.5 ml, according to the measuring method, placed at room temperature 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 h measuring absorbance value, to determine the stability of the forint phenol method. If the absorbance is greater than 1, dilute solution to be tested again. Precision of the experiment: accurate said emblic leafflower fruit wine sample solution 0.5 ml, according to the measuring method of repeated six times. Repetitive experiments: accurate said more than 6 basic wine sample solution 0.5 ml, respectively, according to the measuring method for determining absorbance values. Recovery experiment: the content of known emblic leafflower fruit wine sample solution 3 copies of each precise join gallic acid standard solution 0.5, 1.0, 1.5 ml, according to the method of measuring method, take the average absorbance value and the total phenol content, calculate the sample recovery rate. Gallic acid and quercetin Configuration of the standard solution, precision weigh and gallic acid, respectively 10.0 mg quercetin standard to 25 ml volumetric flask, with 90% methanol concentration of water soluble solvent configured to 400.0 ug/ml single standard solution, shake well, set aside. 25 ml wine extract: take wine, according to the 1:1 ratio to join anhydrous diethyl ether extraction, extraction 3 min, take out ester phase, water phase extraction four times, repeatedly merging ester, less than 35 °C spin steaming to dry, 260 r/min. Residue is dissolved in 2 ml of 90% methanol aqueous solution, 0.45 mu m membrane filter, as the test solution. Linear range: apply different concentration of standard solution, dilute concentrations were 400.0, 200.0, 80.0, 40.0, 20.0, and 8.0 ug/ml of gallic acid, quercetin series of six different concentration of standard solution, precision measuring 10.0 ul into the highly effective liquid phase color spectrometer, sample analysis. Precision experiments: samples from 25 ml wine press wine extraction method, measuring 10 ul sample analysis, repeated sampling 5 times, by chromatographic peak area observation precision index. Repetitive experiments: take 20 ml emblic leafflower fruit wine, add 5 ml, mixed standard solution preparation, according to the fruit wine extraction method to extract for the test solution, respectively, precision measuring 10 ul into the highly effective liquid phase color spectrometer, sample analysis. Solution stability experiment: take the same standard solution and let stand at room temperature, respectively, at 0, 2, 4, 6, 8, 12, 24 hours, measuring 10 ul into the highly effective liquid phase color spectrometer, sample analysis, chromatographic peak area in different time points are calculated separately, and the RSD values, examine the stability of the sample solution. Content of sample recovery rate experiment: take known emblic leafflower fruit wine solution in 3, 20 ml, 5 ml each, respectively, to join the standard solution, preparation of the test solution, measuring 10 ul into the highly effective liquid phase color spectrometer, measuring the recovery rate of the sample. 3 RESULTS AND ANALYSIS 3.1 The establishment of the total phenol standard curve. Accurate quantity from 0.1 mg/ml of gallic acid standard solution 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 ml in 50 ml volumetric flask and add 30 ml deionized water, shake well, add 2.5 ml forint reagent, fully shake, 1 minutes after add 8.0 ml of 9% sodium carbonate solution, blending the capacity. Water bath reaction under 66 degrees Celsius for 10 minutes, 760 nm wavelength determination of absorbance, establish standard curve. Fig.1 The standard curve of total phenol Get regression equation y=0.1322x-0.0099,R=0.9973。 3.2 Used in the determination of total phenol. Accurate according to take more than six basic wine sample solution 0.5 ml, determine the method were determined respectively in the absorbance values. Take the average of the determination of six times, to the total phenol content in the samples of 0.46 mg/ml, total phenol content in fruit wine was 4.6 mg/ml.

The study of phenolics content in the emblica fruit wine