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To observe the Forsen source herbal tea of reserpine in mice model of spleen deficiency of gastrointestinal effects. Mice were injected with reserpine injection to each mice by 0.1mg / kg in 17 days with continuous subcutaneous injection molding, mice dranking water solution respectively at the same time, pills, small dose, small dose of Fukumori Genryo tea, Wang Laoji, will be the corresponding herbal tea instead of mice daily drinking water; the control group was given ordinary drinking water. In 16th, mice were measured the gastrointestinal bioelectric and sacrificed at 17th. To observe the mice’s bioelectricity and organ morphology changes. the spleen deficiency of mice model, compared with the model group, the large and small dose of the Forsen source herbal tea in mice bioelectricity and tissuse morphology without large the influence of the Forsen source herbal tea. No obvious adverse effect on spleen deficiency mice induced by reserpine bioelectricity and organ morphology. Introduction From honeysuckle, licorice, immortality, dried ginger, chrysanthemum, radix puerariae, hawthorn made of Forsen source herbal tea. The herbs are containing components cool. For herbal tea containing more than cool traditional Chinese medicine, whatever the weakness of the spleen and stomach can drinke Forsen source herbal tea.With the increase of modern work pressure, people have irregular diet to gastrointestinal function disorder. Drinking a lot of Forsen source herbal tea whether will lead to the aggravation of the gastrointestinal weak [1], so therefore observed long-term drinking a lot of Forsen source herbal tea in in mice model of spleen deficiency of gastrointestinal. Therefore, we had to observe long-term drinking lots of Forsen source herbal of injection of reserpine induced mice with spleen deficiency model. Experimental Material Pharmaceutical companies to provide cool tea: from honeysuckle, chrysanthemum, grass jelly, licorice, dried ginger, radix puerariae, hawthorn, Chinese yam and other Forsen source herbal tea; Wong Lo Kat herbal tea, batch number 20140901 (specifications 310ml/ bottle); reserpine injection (1ml:1mg), Tianjin Jinyao Pharmaceutical Co., Ltd., batch number 1501091. Gastrointestinal biological apparatus: EGEG-2D type detection apparatus, Hefei Austrian Science and Technology Development Co., Ltd. Kunming mice, male and female, weighing 18 ~ 22g; experimental animal center of Shandong province quality certificate: No 37005413; Experimental methods Take the weight of 18 to 84 22g mice, male and female, were randomly divided into 7 groups, 6 groups of these mice were spleen and deficiency model, another group was control group. Model group were injected with reserpine injection per mouse by 0.1mg /kg in subcutaneous injection, until continuous 17 days. Mice were given 6 groups respectively forsen source a large dose of herbal tea, a small dose of herbal tea (small dose concentration is half of large dose concentration), a large dose of Wang Laoji, a small dose of Wang Laoji (small dose concentration is half of large 2nd International Conference on Machinery, Materials Engineering, Chemical Engineering and Biotechnology (MMECEB 2015) © 2016. The authors Published by Atlantis Press 32 dose concentration), Baohewan aqueous solution that will replace the corresponding herbal tea drinking water of mice; blank group were given normal drinking water. The mice drank corresponding herbal tea instead of drinking water. Mice changed daily drinking water bottle contents every day, continuous 17th. In 16th, mice were anesthetized with ether, in the gastric antrum surface projection of the shearing, 95% B Alcohol degreasing with disking electrode contained saline tampon cover and fixed; The left forelimb wrist as reference electrode and left hind tarsal on as the ground electrode. EGEG-2D gastrointestinal electrographic instrument were recorded 5 min electrogastrogram. Observe the effect on gastrointestinal motility and outcome measures mainly frequency and amplitude. In 17th mice were killed. To put the mice’s stomach (cut along the greater curvature of the stomach and contents make full use of saline wash) and duodenum. Then the stomach and duodenum tissue was placed in 10% neutral buffered formaldehyde solution fixed 24h, making up by slicedand and microscopic examination.

Forsen Herbal Tea in Reserpine in Mice Model of Spleen Deficiency of Gastrointestinal Effects