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The different particle sizes' nano silica spheres are synthesized by the TEOS hydrolysis method. The XRD patterns testified that the sample is single phase and its strongest peak is significantly broadened. From the photos of TEM we can see that the samples' surfaces have a good dispersion and uniform spherical morphology. The Hela cell proliferating activity is assessed by MTT assay, combined with the cell morphology and Annexin Hoechst 33342 staining are employed to determine apoptosis in Hela cells. Results shows that while the Hela cell treated with different concentrations of 200nm nano silica, cytocompatibility becomes better with the lower concentrations of nano silica. 24h cells stained photograph shows that under the concentrations of nano silica 50μg·ml -1 the cell structure is normal and nucleus is complete.

The Preparation and Biological Toxicity Research of the New Controlled Release Carrier Nano-silica