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Objective to compare different ratio of toluidine blue dye solution on the advantages and disadvantages of rat femur dyeing. Methods: the rat femur of materials, fixation, embedding, and sectioning, respectively with different ratio of toluidine blue dye solution dyed, observation of femoral morphology change of the organization. Results show: borax toluidine blue dye solution is better than the other toluidine blue dye solution.

Comparative Evaluation of Four Toluidine Blue Stainings for Rat Femur Effect