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Scorpion venom is usually utilized to induce cancer cell death via apoptosis pathways. Our study focuses on the effects of the Heterometrus liangi scorpion venom on the proliferation of KYSE-510 cells (human esophageal cancer cells) and its related molecular mechanism. Marked morphological changes in KYSE-510 cells after treatment with scorpion venom were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cell apoptosis were determined using flow cytometry (FCM). Moreover, RT-PCR and Western blot analysis was used to investigate the different levels of p21 and caspase-3 expression. Our results showed that scorpion venom at concentrations of 50–100 ug/mL caused dose-dependent inhibition of KYSE-510 cell proliferation. And, the ratio of apoptosis and necrosis increased. Besides, the effect of venom treated in KYSE-510 cells in p21 gene expression was reinforced, but the caspase-3 gene expression had indistinctive effect. The levels of p21 and caspase-3 protein expression were up-regulated after scorpion venom treatment. Therefore, scorpion venom may increase the expressions of p21 and caspase-3 protein to inhibite KYSE-510 proliferation and induce cell apoptosis selectively. Keywords-KYSE-510 cells; gene expression; cell proliferation; cytotoxicity; Heterometrus liangi venom

Cytotoxic and Apoptotic Effects of the Venom of the Scorpion Heterometrus liangi in Human KYSE-510 Cells (Esophageal cancer)