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The article presents the results of three-year studies on microclonal propagation of climbing roses «PalaisRoyal», «Camelot»and «Nahema». At the stage of proliferation the optimum concentration of cytokinin 6benzylaminopurine (6-BAP) in the Murashige and Skoog medium (MSO) for three varieties of roses was 1.0 mg/L. With an increase in the cytokinin content to 2.0 mg/l, a tendency to the proliferation coefficient increase was observed. Positive average correlation dependence of the proliferation coefficient on the size of a cutting was revealed. The use of the commercial product "Siliplant" as trace elements had a positive effect on lengthening of the microcuttings of three rose varieties. The proliferation coefficient was not significantly different from the control one. In order to prepare for the rhizogenesis stage, glucose is the best carbohydrate in the composition of the nutrient medium compared to sucrose, as evidenced by a statistically valid improvement in the quality of the cuttings. The optimal cultivation soil for the adaptation of rose seedlings is a physico-chemical-balanced soil mixture based on highmoor and transitional peat. Keywords—microclonal propagation, climbing roses, growing medium, hormones, adaptation, substrate.

Biotechnology as a heart of innovation in nursery management of ornamental plants